Selection and Validation of Stable Reference Genes in Potato Infected by Pectobacterium atrosepticum Using Real-time Quantitative PCR
Selection and Validation of Stable Reference Genes in Potato Infected by Pectobacterium atrosepticum Using Real-time Quantitative PCR This study delves into the vital role of stable reference genes in the study of potato response to Pectobacterium atrosepticum infection. It identifies and validates the effectiveness of specific genes for accurate normalization in real-time quantitative PCR (RT-qPCR) analysis, which is crucial for measuring gene expression levels in biological research. Understanding the Importance of Reference Genes in Potato Research In the context of molecular plant pathology, the expression stability of candidate reference genes is paramount, especially in assessing the resistance mechanisms in potato varieties infected by Pectobacterium atrosepticum....